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"Jump start and gain" model for dosage compensation in Drosophila based on direct sequencing of nascent transcripts.

ABSTRACT: Dosage compensation in Drosophila is mediated by the MSL complex, which increases male X-linked gene expression approximately 2-fold. The MSL complex preferentially binds the bodies of active genes on the male X, depositing H4K16ac with a 3' bias. Two models have been proposed for the influence of the MSL complex on transcription: one based on promoter recruitment of RNA polymerase II (Pol II), and a second featuring enhanced transcriptional elongation. Here, we utilize nascent RNA sequencing to document dosage compensation during transcriptional elongation. We also compare X and autosomes from published data on paused and elongating polymerase in order to assess the role of Pol II recruitment. Our results support a model for differentially regulated elongation, starting with release from 5' pausing and increasing through X-linked gene bodies. Our results highlight facilitated transcriptional elongation as a key mechanism for the coordinated regulation of a diverse set of genes.

SUBMITTER: Ferrari F 

PROVIDER: S-EPMC3852897 | biostudies-other | 2013 Nov

REPOSITORIES: biostudies-other

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"Jump start and gain" model for dosage compensation in Drosophila based on direct sequencing of nascent transcripts.

Ferrari Francesco F   Plachetka Annette A   Alekseyenko Artyom A AA   Jung Youngsook L YL   Ozsolak Fatih F   Kharchenko Peter V PV   Park Peter J PJ   Kuroda Mitzi I MI  

Cell reports 20131031 3

Dosage compensation in Drosophila is mediated by the MSL complex, which increases male X-linked gene expression approximately 2-fold. The MSL complex preferentially binds the bodies of active genes on the male X, depositing H4K16ac with a 3' bias. Two models have been proposed for the influence of the MSL complex on transcription: one based on promoter recruitment of RNA polymerase II (Pol II), and a second featuring enhanced transcriptional elongation. Here, we utilize nascent RNA sequencing to  ...[more]