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Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered Escherichia coli.

ABSTRACT: This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an ?-fluoro-?-keto ester, a bis-trifluoromethylated acetophenone, and a symmetrical ?-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to preparative-scale dehydrogenase-catalyzed reactions successfully.

SUBMITTER: Dascier D 

PROVIDER: S-EPMC4105172 | biostudies-other | 2014 Jun

REPOSITORIES: biostudies-other

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Influence of Cofactor Regeneration Strategies on Preparative-Scale, Asymmetric Carbonyl Reductions by Engineered <i>Escherichia coli.</i>

Dascier Dimitri D   Kambourakis Spiros S   Hua Ling L   Rozzell J David JD   Stewart Jon D JD  

Organic process research & development 20140217 6

This study was designed to determine whether whole cells or crude enzyme extracts are more effective for preparative-scale ketone reductions by dehydrogenases as well as learning which cofactor regeneration scheme is most effective. Based on results from three representative ketone substrates (an α-fluoro-β-keto ester, a <i>bis</i>-trifluoromethylated acetophenone, and a symmetrical β-diketone), our results demonstrate that several nicotinamide cofactor regeneration strategies can be applied to  ...[more]