Unknown

Dataset Information

0

Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.


ABSTRACT: Transcription factor TFIIIB plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. We report the purification of a human TFIIIB-derived complex containing only the TATA-binding polypeptide (TBP) and a 90-kDa subunit (TFIIIB90) and the isolation of a cDNA clone encoding the 90-kDa subunit. The N-terminal half of TFIIIB90 exhibits sequence similarity to the yeast TFIIIB70 (BRF) and the class II transcription factor TFIIB and interacts weakly with TBP. The C-terminal half of TFIIIB90 contains a high-mobility-group protein 2 (HMG2)-related domain and interacts strongly with TBP. Recombinant TFIIIB90 plus recombinant human TBP substitute for human TFIIIB in a complementation assay for transcription of 5S, tRNA, and VA1 RNA genes, and both the TFIIB-related domain and the HMG2-related domain are required for this activity. TFIIIB90 is also required for transcription of human 7SK and U6 RNA genes by RNA polymerase III, but apparently within a complex distinct from the TBP/TFIIIB90 complex.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC41464 | biostudies-other | 1995 Jul

REPOSITORIES: biostudies-other

altmetric image

Publications

Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.

Wang Z Z   Roeder R G RG  

Proceedings of the National Academy of Sciences of the United States of America 19950701 15


Transcription factor TFIIIB plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. We report the purification of a human TFIIIB-derived complex containing only the TATA-binding polypeptide (TBP) and a 90-kDa subunit (TFIIIB90) and the isolation of a cDNA clone encoding the 90-kDa subunit. The N-terminal half of TFIIIB90 exhibits sequence similarity to the yeast TFIIIB70 (BRF) and the class II transcription factor T  ...[more]

Similar Datasets

| S-EPMC316744 | biostudies-literature
| S-EPMC8317121 | biostudies-literature
| S-EPMC4419465 | biostudies-literature
| S-EPMC84305 | biostudies-literature
| S-EPMC2841526 | biostudies-literature
| S-EPMC2885798 | biostudies-literature
| S-EPMC8310312 | biostudies-literature
| S-EPMC2771086 | biostudies-literature
| S-EPMC4038581 | biostudies-literature
| S-EPMC3255765 | biostudies-literature