Ontology highlight
ABSTRACT:
SUBMITTER: Kienzle C
PROVIDER: S-EPMC4151145 | biostudies-other | 2014 Sep
REPOSITORIES: biostudies-other
The Journal of cell biology 20140901 5
The actin filament severing protein cofilin-1 (CFL-1) is required for actin and P-type ATPase secretory pathway calcium ATPase (SPCA)-dependent sorting of secretory proteins at the trans-Golgi network (TGN). How these proteins interact and activate the pump to facilitate cargo sorting, however, is not known. We used purified proteins to assess interaction of the cytoplasmic domains of SPCA1 with actin and CFL-1. A 132-amino acid portion of the SPCA1 phosphorylation domain (P-domain) interacted w ...[more]