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Lab-on-a-brain: implantable micro-optical fluidic devices for neural cell analysis in vivo.

ABSTRACT: The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon laser scanning microscopy and the microfluidic delivery of chemicals or drugs directly into the brain. A proof-of-concept experiment of single-synapse stimulation by two-photon uncaging of caged glutamate and observation of dendritic spine shrinkage over subsequent days demonstrated a promising use for the present technology.

SUBMITTER: Takehara H 

PROVIDER: S-EPMC4205880 | biostudies-other | 2014

REPOSITORIES: biostudies-other

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Lab-on-a-brain: implantable micro-optical fluidic devices for neural cell analysis in vivo.

Takehara Hiroaki H   Nagaoka Akira A   Noguchi Jun J   Akagi Takanori T   Kasai Haruo H   Ichiki Takanori T  

Scientific reports 20141022

The high-resolution imaging of neural cells in vivo has brought about great progress in neuroscience research. Here, we report a novel experimental platform, where the intact brain of a living mouse can be studied with the aid of a surgically implanted micro-optical fluidic device; acting as an interface between neurons and the outer world. The newly developed device provides the functions required for the long-term and high-resolution observation of the fine structures of neurons by two-photon  ...[more]