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Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence.

ABSTRACT: Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the Gi class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal strains. Significantly, the resulting transgenic fungal strains were also hypovirulent. These results indicate a crucial role for G-protein-linked signal transduction in fungal pathogenesis and suggest a molecular basis for virus-mediated attenuation of fungal virulence.

SUBMITTER: Choi GH 

PROVIDER: S-EPMC42867 | biostudies-other | 1995 Jan

REPOSITORIES: biostudies-other

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Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence.

Choi G H GH   Chen B B   Nuss D L DL  

Proceedings of the National Academy of Sciences of the United States of America 19950101 1

Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the Gi class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal  ...[more]