Impact of splenic artery ligation after major hepatectomy on liver function, regeneration and viability.

ABSTRACT: It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24?h, induces a relative decrease in oxidative stress during the first 48?hours, allows antioxidant capacity increment after 24?h, which is reflected in a decrease of half-time normalized liver curve at 48?h and at 72?h and in an increase of mitotic index between 48?h and 72?h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48?hours.

SUBMITTER: Carrapita J 

PROVIDER: S-EPMC5057079 | biostudies-other | 2016 Oct

REPOSITORIES: biostudies-other