TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization.

ABSTRACT: Top-down mass spectrometry enables the observation of whole complex proteoforms in biological samples and provides crucial information complementary to bottom-up mass spectrometry. Because of the complexity of top-down mass spectra and proteoforms, it is a challenging problem to efficiently interpret top-down tandem mass spectra in high-throughput proteome-level proteomics studies. We present TopPIC, a tool that efficiently identifies and characterizes complex proteoforms with unknown primary structure alterations, such as amino acid mutations and post-translational modifications, by searching top-down tandem mass spectra against a protein database.http://proteomics.informatics.iupui.edu/software/toppic/ CONTACT: [e-mail] information: Supplementary data are available at Bioinformatics online.

SUBMITTER: Kou Q 

PROVIDER: S-EPMC5181555 | biostudies-other | 2016 Nov

REPOSITORIES: biostudies-other