Risk stratification of adult T-cell leukemia/lymphoma using immunophenotyping.

ABSTRACT: Adult T-cell leukemia/lymphoma (ATL), a human T-lymphotropic virus type 1 (HTLV-1)-associated disease, has a highly variable clinical course and four subtypes with therapeutic and prognostic implications. However, there are overlapping features between ATL subtypes and between ATL and nonmalignant (non-ATL) HTLV-1 infection complicating diagnosis and prognostication. To further refine the diagnosis and prognosis of ATL, we characterized the immunophenotype of HTLV-1-infected cells in ATL and non-ATL. A retrospective study of peripheral blood samples from 10 HTLV-1-uninfected subjects (UI), 54 HTLV-1-infected patients with non-ATL, and 22 with ATL was performed using flow cytometry. All patients with ATL had CD4+  CCR4+  CD26- immunophenotype and the frequency of CD4+  CCR4+  CD26- T cells correlated highly significantly with the proviral load in non-ATL suggesting CD4+  CCR4+  CD26- as a marker of HTLV-1-infected cells. Further immunophenotyping of CD4+  CCR4+   CD26- cells revealed that 95% patients with ATL had a CD7- (?30% CD7+ cells), whereas 95% HTLV+ non-ATL had CD7+ (>30% CD7+ cells) immunophenotype. All patients with aggressive ATL had a CCR7+ (?30%), whereas 92% with indolent ATL and 100% non-ATL had a CCR7- (<30%) immunophenotype. Patients with nonprogressing indolent ATL were CD127+ but those with progressive lymphocytosis requiring systemic therapy had a CD127- (?30%) immunophenotype. In summary, HTLV-1-infected cells have a CD4+  CCR4+  CD26- immunophenotype. Within this population, CD7- phenotype suggests a diagnosis of ATL, CCR7+ phenotype identifies aggressive ATL, while CCR7- CD127- phenotype identifies progressive indolent ATL.

SUBMITTER: Kagdi HH 

PROVIDER: S-EPMC5269699 | biostudies-other | 2017 Jan

REPOSITORIES: biostudies-other