Ontology highlight
ABSTRACT:
SUBMITTER: Kim H
PROVIDER: S-EPMC5316869 | biostudies-other | 2017 Feb
REPOSITORIES: biostudies-other
Nature communications 20170216
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of recombinant Cpf1 proteins with in vitro transcribed or chemically synthesized target-specific crRNAs into protoplasts isolated from soybean and wild tobacco. Designed crRNAs are unique and do not hav ...[more]