Ontology highlight
ABSTRACT:
SUBMITTER: Lu S
PROVIDER: S-EPMC5465213 | biostudies-other | 2017 Jun
REPOSITORIES: biostudies-other
Lu Shan S Shen Xiaorui X Chen Baoshan B
Scientific reports 20170608 1
Sporisorium scitamineum is the causative agent responsible for smut disease of sugarcane worldwide. However, lack of efficient gene manipulation system makes this fungus much behind the type model of the smut fungi in molecular biology. Here, we report the development of a CRISPR/Cas9 and T-DNA based dual vector system that allowed efficient knock-out or knock-in of a gene of interest in the S. scitamineum in a site-specific manner. By using Mfa2, a key player in the mating event in S. scitamine ...[more]