Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.

ABSTRACT: Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.

SUBMITTER: Kurauskas V 

PROVIDER: S-EPMC5551963 | biostudies-other | 2017

REPOSITORIES: biostudies-other