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TGF-?1 Negatively Regulates the Number and Function of Hematopoietic Stem Cells.

ABSTRACT: Transforming growth factor ?1 (TGF-?1) plays a role in the maintenance of quiescent hematopoietic stem cells (HSCs) in vivo. We asked whether TGF-?1 controls the cell cycle status of HSCs in vitro to enhance the reconstitution activity. To examine the effect of TGF-?1 on the HSC function, we used an in vitro culture system in which single HSCs divide with the retention of their short- and long-term reconstitution ability. Extensive single-cell analyses showed that, regardless of its concentration, TGF-?1 slowed down the cell cycle progression of HSCs but consequently suppressed their self-renewal potential. Cycling HSCs were not able to go back to quiescence with TGF-?1. This study revealed a negative role of TGF-?1 in the regulation of the HSC number and reconstitution activity.

SUBMITTER: Wang X 

PROVIDER: S-EPMC6067088 | biostudies-other | 2018 Jul

REPOSITORIES: biostudies-other

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TGF-β1 Negatively Regulates the Number and Function of Hematopoietic Stem Cells.

Wang Xiaofang X   Dong Fang F   Zhang Sen S   Yang Wanzhu W   Yu Wenying W   Wang Zhao Z   Zhang Shanshan S   Wang Jinhong J   Ma Shihui S   Wu Peng P   Gao Yun Y   Dong Ji J   Tang Fuchou F   Cheng Tao T   Ema Hideo H  

Stem cell reports 20180621 1

Transforming growth factor β1 (TGF-β1) plays a role in the maintenance of quiescent hematopoietic stem cells (HSCs) in vivo. We asked whether TGF-β1 controls the cell cycle status of HSCs in vitro to enhance the reconstitution activity. To examine the effect of TGF-β1 on the HSC function, we used an in vitro culture system in which single HSCs divide with the retention of their short- and long-term reconstitution ability. Extensive single-cell analyses showed that, regardless of its concentratio  ...[more]

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