Enantiomer discrimination in ?-phenylalanine degradation by a newly isolated Paraburkholderia strain BS115 and type strain PsJN.

ABSTRACT: Despite their key role in numerous natural compounds, ?-amino acids have rarely been studied as substrates for microbial degradation. Fermentation of the newly isolated Paraburkholderia strain BS115 and the type strain P. phytofirmans PsJN with ?-phenylalanine (?-PA) as sole nitrogen source revealed (S)-selective transamination of ?-PA to the corresponding ?-keto acid by both strains, accompanied by substantial formation of acetophenone (AP) from spontaneous decarboxylation of the emerging ?-keto acid. While the PsJN culture became stationary after entire (S)-?-PA consumption, BS115 showed further growth at a considerably slower rate, consuming (R)-?-PA without generation of AP which points to a different degradation mechanism for this enantiomer. This is the first report on degradation of both enantiomers of any ?-amino acid by one single bacterial strain.

SUBMITTER: Buß O 

PROVIDER: S-EPMC6150868 | biostudies-other | 2018 Sep

REPOSITORIES: biostudies-other