Project description:HCC1954 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1477 | LINCS

iMN (Exp 2) - ALS, SMA and Control (unaffected) iMN cell lines differentiated from iPS cell lines using a long differentiation protocol - ATAC-seq

Project description:ATAC-Seq was carried out on isolated nuclei obtained from induced Pluripotnent Stem Cells (iPSC) cell lines. These lines were derived from ALS, SMA and Control (unaffected) individuals (three of each).

2017-05-01 | LDS-1400 | LINCS

LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 11 of 15: Time-dependent mean normalized growth rate inhibition values for biological replicate 2.

Project description: Not available

2017-05-12 | LDS-1418 | LINCS

LINCS MCF 10A Common Project: Rolling-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 15 of 15: Time-dependent dose-response metrics for biological replicate 3.

Project description: Not available

2017-05-12 | LDS-1422 | LINCS

MEMA HMEC240L Staining Set 1

Project description:HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1435 | LINCS

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