Using CombiCells, a platform for titration and combinatorial display of cell surface ligands, to study T-cell antigen sensitivity modulation by accessory receptors

ABSTRACT: Understanding cellular decisions due to receptor-ligand interactions at cell-cell interfaces has been hampered by the difficulty of independently varying the surface density of multiple different ligands. Here, we express the synthetic binder protein SpyCatcher, designed to form spontaneous covalent bonds with interactors carrying a Spytag, on the cell surface. Using this, we show that addition of different concentrations and combinations of native Spytag-fused ligands allows for the combinatorial display of ligands on cells within minutes. We use this combinatorial display of cell surface ligands -called CombiCells- to assess T-cell antigen sensitivity and the impact of T-cell co-stimulation and co-inhibition receptors. We find that the T-cell receptor (TCR) displayed greater sensitivity

SUBMITTER: Dr. Ashna Patel 

PROVIDER: S-SCDT-10_1038-S44318-023-00012-1 | biostudies-other |

REPOSITORIES: biostudies-other