A subset of Megakaryocytes Regulates Development of Hematopoietic Stem Cell Precursors

ABSTRACT: Understanding the regulatory mechanisms facilitating hematopoietic stem cell (HSC) specification during embryogenesis is important for the generation of HSCs in vitro. Megakaryocyte emerged from the yolk sac and produce platelets, which are involved in multiple biological processes, such as preventing hemorrhage. However, whether megakaryocytes regulate HSC development in the embryonic aorta-gonad-mesonephros (AGM) region is unclear. Here, we use platelet factor 4 (PF4)-Cre;Rosa-tdTomato+ cells to report presence of megakaryocytes in the HSC developmental niche. Further, we use a PF4-Cre;Rosa-DTA(DTA) depletion model to reveal that megakaryocytes control HSC specification in the mouse embryos. Megakaryocyte deficiency blocks the generation and maturation of pre-HSCs and alters HSC activity

SUBMITTER: Zhuan Li 

PROVIDER: S-SCDT-10_1038-S44318-024-00079-4 | biostudies-other |

REPOSITORIES: biostudies-other