LATS1 controls CTCF chromatin occupancy and hormonal response of 3D-grown breast cancer cells

ABSTRACT: The cancer epigenome has been studied in cells cultured in two-dimensional (2D) monolayers, but recent studies highlight the impact of the extracellular matrix and the three-dimensional (3D) environment on multiple cellular functions. Here, we report the physical, biochemical, and genomic differences between T47D breast cancer cells cultured in 2D and as 3D spheroids. Cells within 3D spheroids exhibit a rounder nucleus with less accessible, more compacted chromatin, as well as altered expression of ~2,000 genes, the majority of which become repressed. Hi-C analysis reveals that cells in 3D are enriched for regions belonging to the B-compartment, have decreased chromatin-bound CTCF and increased fusion of topologically associating domains (TADs). Upregulation of the Hippo pathway in 3D sphe

SUBMITTER: Dr. Julieta, Ramirez Cuellar 

PROVIDER: S-SCDT-10_1038-S44318-024-00080-X | biostudies-other |

REPOSITORIES: biostudies-other