TEAD2 initiates ground-state pluripotency by mediating chromatin looping

ABSTRACT: The transition of mouse embryonic stem cells (ESCs) between serum/LIF and 2i(MEK and GSK3 kinase inhibitor)/LIF culture conditions serves as a valuable model for exploring the mechanisms underlying ground and confused pluripotent states. Regulatory networks comprising core and ancillary pluripotency factors drive the gene expression programs defining stable naïve pluripotency. In our study, we systematically screened factors essential for ESC pluripotency, identifying TEAD2 as an ancillary factor maintaining ground-state pluripotency in 2i/LIF ESCs and facilitating the transition from serum/LIF to 2i/LIF ESCs. TEAD2 exhibits increased binding to chromatin in 2i/LIF ESCs, targeting active chromatin regions to regulate the expression of 2i-specific genes. Additionally, TEAD2 facilitates the

SUBMITTER: Rong Guo 

PROVIDER: S-SCDT-10_1038-S44318-024-00086-5 | biostudies-other |

REPOSITORIES: biostudies-other