Transcriptional rewiring of an evolutionarily conserved circadian clock

ABSTRACT: Circadian clocks temporally coordinate daily organismal biology over the 24-hour cycle. Their molecular design, preserved between fungi and animals, is based on a core-oscillator composed of a one-step transcriptional-translational-negative-feedback-loop (TTFL). To test whether this evolutionarily conserved TTFL architecture is the only plausible way for achieving a functional circadian clock, we adopted a transcriptional rewiring approach, artificially co-opting regulators of the circadian output pathways into the core-oscillator. Herein we describe one of these semi-synthetic clocks which maintains all basic circadian features but, notably, it also exhibits new attributes such as a "lights-on timer" logic, where clock phase is fixed at the end of the night. Our findings indicate that fun

SUBMITTER: Alejandra Goity 

PROVIDER: S-SCDT-10_1038-S44318-024-00088-3 | biostudies-other |

REPOSITORIES: biostudies-other