NON-VESICULAR PHOSPHATIDYLINOSITOL TRANSFER PLAYS CRITICAL ROLES IN DEFINING ORGANELLE LIPID COMPOSITION

ABSTRACT: Phosphatidylinositol (PI) is the precursor lipid for the minor phosphoinositides (PPIns), which are critical for multiple functions in all eukaryotic cells. It is poorly understood how phosphatidylinositol, which is synthesized in the ER, reaches those membranes where PPIns are formed. Here, we used VT01454, a recently identified inhibitor of class I PI transfer proteins (PITPs), to unravel their roles in lipid metabolism, and solved the structure of inhibitor-bound PITPNA to gain insight into the mode of inhibition. We found that class I PITPs not only distribute PI for PPIns production in various organelles such as the plasma membrane (PM) and late endosomes/lysosomes, but that their inhibition also significantly reduced the levels of phosphatidylserine, di- and triacylglycerols, and oth

SUBMITTER: Yeun Ju Kim 

PROVIDER: S-SCDT-10_1038-S44318-024-00096-3 | biostudies-other |

REPOSITORIES: biostudies-other