Spatially distinct epithelial and mesenchymal cell subsets along progressive lineage restriction in the branching embryonic mammary gland

ABSTRACT: How cells coordinate morphogenetic cues and fate specification during development remains a fundamental question in organogenesis. The mammary gland arises from multipotent stem cells (MaSCs), which are progressively replaced by unipotent progenitors by birth. However, the lack of specific markers for early fate specification has prevented the delineation of the features and spatial localization of MaSC-derived lineage-committed progenitors. Here, using single-cell RNA-sequencing from E13.5 to birth, we produced an atlas of matched mouse mammary epithelium and mesenchyme and reconstructed the differentiation trajectories of MaSCs towards basal and luminal fate. We show that murine MaSCs exhibit lineage commitment at E15.5, just prior to the first sprouting events of mammary branching morph

SUBMITTER: Dr. Claudia Carabaña 

PROVIDER: S-SCDT-10_1038-S44318-024-00115-3 | biostudies-other |

REPOSITORIES: biostudies-other