Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions

ABSTRACT: Endothelial cell responses to fluid shear stress from blood flow are crucial for vascular development, function and disease. A complex of PECAM-1, VE-cadherin, VEGF receptors (VEGFRs) and PlexinD1 located at cell-cell junctions mediates many of these events. But available evidence suggests that another mechanosensor upstream of PECAM-1 initiates signaling. Hypothesizing that GPCR and Gα proteins may serve this role, we performed siRNA screening of Gα subunits and found that Gαi2 and Gαq/11 are required for activation of the junctional complex. We then developed a new activation assay, which showed that these G proteins are activated by flow. We next mapped the Gα residues required for activation and developed an affinity purification method that used this information to identify latrophili

SUBMITTER: Keiichiro Tanaka 

PROVIDER: S-SCDT-10_1038-S44318-024-00142-0 | biostudies-other |

REPOSITORIES: biostudies-other