RNA fine-tunes estrogen receptor-alpha binding on low-affinity DNA motifs for transcriptional regulation

ABSTRACT: Transcription factors (TFs) regulate gene expression by binding, with varying strength, to DNA via their DNA-binding domain. Additionally, some TFs also interact with RNA, which modulates transcription factor binding to chromatin. However, whether RNA-mediated TF binding results in differential transcriptional outcomes remains unknown. In this study, we demonstrate that estrogen receptor α (ERα), a ligand-activated TF, interacts with RNA in a ligand-dependent manner. Defects in RNA binding lead to genome-wide loss of ERα recruitment, particularly at weaker ERα-motifs. Furthermore, ERα mobility in the nucleus increases in the absence of its RNA-binding capacity. Unexpectedly, this increased mobility coincides with robust polymerase loading and transcription of ERα-regulated genes that harbo

SUBMITTER: Deepanshu Soota 

PROVIDER: S-SCDT-10_1038-S44318-024-00225-Y | biostudies-other |

REPOSITORIES: biostudies-other