Sex-specific regulatory architecture of pancreatic islets from subjects with and without type 2 diabetes

ABSTRACT: Patients with type 2 and type 1 diabetes (T2D and T1D) exhibit sex-specific differences in insulin secretion, the mechanisms of which are unknown. We examined sex differences in human pancreatic islets from 52 donors with and without T2D combining single cell RNA-sequencing (scRNA-seq) and single nucleus ATAC-sequencing (snATAC-seq) with assays probing hormone secretion and bioenergetics. In nondiabetic (ND) donors, sex differences in islet cell chromatin accessibility and gene expression predominantly involved sex chromosomes. In contrast, islets from T2D donors exhibited similar sex differences in sex chromosome-encoded differentially expressed genes (DEGs) as ND donors, but also exhibited sex differences in autosomal genes. Comparing β cells from T2D and ND donors, gene enrichment of fe

SUBMITTER: Dr. Franck Mauvais-Jarvis 

PROVIDER: S-SCDT-10_1038-S44318-024-00313-Z | biostudies-other |

REPOSITORIES: biostudies-other