Partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion

ABSTRACT: Biogenesis of membrane-bound organelles involves the synthesis, remodelling, and degradation of their constituent phospholipids. How these pathways regulate organelle size remains poorly understood. Here we demonstrate that a lipid-degradation pathway inhibits expansion of the endoplasmic reticulum (ER) membrane. Phospholipid diacylglycerol acyltransferases (PDATs) use endogenous phospholipids as fatty-acyl donors to generate triglyceride stored in lipid droplets. The significance of this non-canonical triglyceride biosynthesis pathway has remained elusive. We find that the activity of the yeast PDAT Lro1 is regulated by a membrane-proximal helical segment facing the luminal side of the ER bilayer. To reveal the biological roles of PDATs, we engineered an Lro1 variant with derepressed acti

SUBMITTER: Pawel, K Lysyganicz 

PROVIDER: S-SCDT-10_1038-S44318-024-00355-3 | biostudies-other |

REPOSITORIES: biostudies-other