A novel biosensor for the spatiotemporal analysis of STING activation during innate immune responses to dsDNA

ABSTRACT: The cGAS-STING signalling pathway has a central role in the innate immune response to extrinsic and intrinsic sources of cytoplasmic dsDNA. At the core of this pathway is cGAS-dependent production of the intra- and extra-cellular messenger cGAMP, which activates STING and leads to IRF3-dependent expression of cytokines and interferons. Despite its relevance to viral and bacterial infections, cell death, and genome instability, the lack of specific live-cell reporters has precluded spatiotemporal analyses of cGAS-STING signalling. Here, we generate a fluorescent biosensor termed SIRF (STING-IRF3), which reports on the functional interaction between activated STING and IRF3 at the Golgi. We show that cells harboring SIRF react in a time- and concentration-dependent manner both to STING agoni

SUBMITTER: Steve Smarduch 

PROVIDER: S-SCDT-10_1038-S44318-025-00370-Y | biostudies-other |

REPOSITORIES: biostudies-other