The Structure of Basal Body Inner Junctions from Tetrahymena Revealed by Electron Cryo-Tomography

ABSTRACT: The cilium is a microtubule-based eukaryotic organelle critical for many cellular functions. Its assembly initiates at a basal body and continues as an axoneme that projects out of the cell to form a functional cilium. This assembly process is tightly regulated. However, our knowledge of the molecular architecture and the mechanism of assembly is limited. By applying cryo-electron tomography, we obtained structures of the inner junction in three regions of the cilium from Tetrahymena: the proximal, the central core of basal body, and the axoneme. We identified several protein components in the basal body. While a few proteins are distributed throughout the entire length of the organelle, many are restricted to specific regions, forming intricate local interaction networks in the inner junc

SUBMITTER: Sam Li 

PROVIDER: S-SCDT-10_1038-S44318-025-00392-6 | biostudies-other |

REPOSITORIES: biostudies-other