Oligomerised RIPK1 is the main core component of the CD95 necrosome

ABSTRACT: The necrosome is the key macromolecular signalling platform initiating necroptosis, i.e., a RIPK1/RIPK3-dependent programme of cell death with an important role in the control of inflammation in multicellular organisms. However, the composition and structure of the necrosome remain incompletely understood. Here we use biochemical assays, quantitative mass spectrometry, and AlphaFold modelling to decipher the composition and derive a structural model of the CD95L/BV6-induced necrosome. We identify RIPK1 as the central component of the necrosome, forming the core of this complex. In addition, AlphaFold modelling provides insights into the structural mechanisms underlying RIPK1 oligomerization, highlighting the critical role of type-II interactions between the Death Domains (DDs) of FADD and

SUBMITTER: Nikita Ivanisenko 

PROVIDER: S-SCDT-10_1038-S44318-025-00433-0 | biostudies-other |

REPOSITORIES: biostudies-other