Master transcription-factor binding sites constitute the core of early replication control elements

ABSTRACT: Eukaryotic genomes replicate in a defined temporal order called the replication timing (RT) program. Replication timing is developmentally regulated with potential to drive cell fate transitions, but mechanisms controlling replication timing remain elusive. We previously identified "Early Replication Control Elements" (ERCEs) necessary for early RT, domain-wide transcription, 3D chromatin architecture and compartmentalization in mouse embryonic stem cells (mESCs), but deletions identifying ERCEs were large and encompassed many putative regulatory elements. Here, we show that ERCEs are compound elements, whose RT activity can largely be accounted for by multiple bind-ing sites of diverse master transcription factors (subERCEs), distinguished from other such sites by their long-range interac

SUBMITTER: Jesse, Lloyd Turner 

PROVIDER: S-SCDT-10_1038-S44318-025-00501-5 | biostudies-other |

REPOSITORIES: biostudies-other