Bacterial cell widening alters periplasmic size and activates envelope stress responses

ABSTRACT: The Rcs signal transduction system is a phosphorelay responsible for sensing enterobacterial cell envelope stresses. In Escherichia coli, the Rcs system is required to survive treatment with A22 and mecillinam, antibiotics that perturb cell size. To test whether size changes are correlated with envelope damage and thereby sensed by the Rcs system, we tuned E. coli cell size via A22 treatment, mutations in the cell-shape determinant MreB, and mechanically-confined growth. In all conditions, cell width was strongly correlated with Rcs activation, and RcsF, the outer-membrane-localized upstream component, was essential for responding to cell width changes. Several gene deletions that induce Rcs resulted in cells that were wider than wild-type. Cryo-electron microscopy revealed that the peripl

SUBMITTER: Kerwyn, Casey Huang 

PROVIDER: S-SCDT-10_1038-S44318-025-00534-w | biostudies-other |

REPOSITORIES: biostudies-other