Functional landscape of ubiquitin linkages connects K29-linked ubiquitylation to epigenome integrity

ABSTRACT: Ubiquitin chains with distinct linkages govern the outcome of numerous critical ubiquitin-dependent signaling processes, but their particular targets and functional impacts remain incompletely understood due to a paucity of tools for their specific detection and manipulation. Here, we applied a cell-based ubiquitin replacement strategy, which allows targeted conditional abrogation of each of the seven lysine-based ubiquitin linkages in human cells, to profile system-wide impacts of disabling formation of individual chain types. This revealed proteins and processes regulated by each of these poly-ubiquitin topologies, as well as indispensable roles of K48-, K63- and K27-linkages in cell proliferation. We show that K29-linked ubiquitylation is strongly associated with chromosome biology, and

SUBMITTER: Javier Arroyo-Gomez 

PROVIDER: S-SCDT-10_1038-S44318-025-00599-7 | biostudies-other |

REPOSITORIES: biostudies-other