Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens

ABSTRACT: T-cells use their T-cell receptors (TCRs) to discriminate between higher-affinity foreign and lower-affinity self-peptide-MHC (pMHC) antigen complexes. The OT-I mouse TCR is widely used to study antigen discrimination between foreign and self-pMHC antigens, and previous work suggested it achieved near-perfect discrimination between higher- and lower-affinity antigens. However, other TCRs show imperfect discrimination. To resolve these discrepancies, we developed in this study a protocol for measuring ultra-low TCR-pMHC binding affinities to determine the 3D solution affinities of OT-I TCR for 19 pMHCs. These revised 3D affinities now strongly correlate with 2D membrane affinities and predict T-cell functional responses. Our results indicate that OT-I exhibits enhanced yet imperfect discrim

SUBMITTER: Anna Huhn 

PROVIDER: S-SCDT-10_1038-S44318-025-00644-5 | biostudies-other |

REPOSITORIES: biostudies-other