Ubiquitin pathway blockade reveals endogenous ADP-ribosylation marking PARP7 and AHR for degradation

ABSTRACT: ADP-ribosylation is an important protein post-translational modification catalysed by a family of PARP enzymes in humans, and among other processes is involved in DNA damage responses and immunity. While poly-ADP-ribosylation has been established as a protein-degradation signal in several cases, the role of mono-ADP-ribosylation in protein turnover has remained elusive and mostly supported by overexpression systems. Here, we describe a way to visualise high levels of endogenous ADP-ribosylation by inhibiting the ubiquitin pathway. By blocking ubiquitylation/proteasome activity, we found that ADP-ribosylation via at least three different PARPs (PARP7, PARP1 and TNKS) can be greatly induced. We discovered that specific activation of the aryl hydrocarbon receptor (AHR) pathway in combination

SUBMITTER: Andrii Gorelik 

PROVIDER: S-SCDT-10_1038-S44318-025-00656-1 | biostudies-other |

REPOSITORIES: biostudies-other