Conserved shifts in sperm small non-coding RNA profiles during mouse and human aging

ABSTRACT: Sperm aging impacts male fertility and offspring health, highlighting the need for reliable aging biomarkers to guide reproductive decisions. However the molecular determinants of sperm fitness during aging remain ill-defined. Here, we profiled sperm small non-coding RNAs (sncRNAs) using PANDORA-seq, which overcomes RNA modification-induced detection bias to capture previously undetectable sncRNA species associated with mouse and human spermatocytes throughout the lifespan. We identified an "aging cliff" in mouse sperm RNA profiles-a sharp age-specific transition marked by significant shifts in genomic and mitochondrial tRNA-derived small RNAs (tsRNAs) and rRNA-derived small RNAs (rsRNAs). Notably, rsRNAs in sperm heads exhibited a transformative length shift, with longer rsRNAs increasing

SUBMITTER: Junchao Shi 

PROVIDER: S-SCDT-10_1038-S44318-025-00687-8 | biostudies-other |

REPOSITORIES: biostudies-other