Arthropod exosomal glycine-rich protein as a potential vaccine candidate

ABSTRACT: During blood feeding, Ixodidae ticks secrete cement proteins, including glycine-rich proteins (GRPs), that facilitate attachment to the vertebrate host. However, the molecular mechanisms underlying exosomal GRP secretion at the feeding site and their roles in tick-pathogen interactions remain poorly understood. Here, we analyzed the Ixodes scapularis genome to identify salivary exosomal components involved in modulation of the tick-host skin interface. We identify an arthropod exosomal GRP (XM_002400035) that promotes transmission of Langat virus (LGTV), a tick-borne flavivirus, from ticks to vertebrate hosts. XM_002400035 was consistently upregulated in LGTV-infected I. scapularis ticks, tick-derived cells, and in tick exosomes. RNAi-mediated silencing of this exosomal GRP reduced viral l

SUBMITTER: Waqas Ahmed 

PROVIDER: S-SCDT-10_1038-S44318-026-00709-Z | biostudies-other |

REPOSITORIES: biostudies-other