Lipopolysaccharide confinement in the bacterial outer membrane is governed by interactions within the conserved Lipid A anchor

ABSTRACT: The outer membrane of Gram-negative bacteria is an asymmetric bilayer in which lipopolysaccharide (LPS), the principal component of the outer leaflet, promotes tight packing and ordering of the membrane components that are essential for the barrier and load-bearing functions of this membrane. Lipopolysaccharide mobility is known to be restricted in the outer membrane, but this confinement and the underlying biophysical interactions responsible remain to be fully characterized. Here, we apply a bio-orthogonal strategy for in situ site-specific fluorescent labeling of LPS. Using fluorescence microscopy, we quantify LPS lateral confinement in the outer membrane of Escherichia coli and demonstrate that this confinement is independent of oligosaccharide domain structure. We show that lipopolysa

SUBMITTER: Joe Nabarro 

PROVIDER: S-SCDT-10_1038-S44318-026-00711-5 | biostudies-other |

REPOSITORIES: biostudies-other