Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potential

ABSTRACT: Naive human pluripotent stem cells (hPSCs) represent a pre-implantation epiblast state able to efficiently differentiate into embryonic and extraembryonic pre-implantation lineages and to self-organise in vitro into blastocyst-like structures called blastoids. Naive hPSC maintenance routinely relies on co-culture with mouse embryonic fibroblast (MEFs) as feeder cells, a method prone to variability and analytical confounders. Here, we describe a feeder-free culture system based on serum coating that supports long-term maintenance of naive hPSC. Across five laboratories, 30 serum batches were evaluated for the expansion of eight naive hPSC lines for up to 25 passages. Mass spectrometry analysis identified fibronectin and collagens as extracellular matrix proteins consistently present in seru

SUBMITTER: Giada Rossignoli 

PROVIDER: S-SCDT-10_1038-S44318-026-00714-2 | biostudies-other |

REPOSITORIES: biostudies-other