Hybrid endosomal coats contain different classes of sorting nexins

ABSTRACT: Endosomes are protein sorting stations, where multiple membrane coats form tubulovesicular carriers exporting proteins to the Golgi, the plasma membrane, or endo-lysosomal compartments. Distinct classes of sorting nexins are assumed to form distinct homogeneous coats that define the endosomal sorting routes and their cargos. Snx3 and the SNX-BAR proteins Vps5-Vps17 belong to different sorting-nexin classes. They can form homogeneous retromer-dependent coats that differ in structure and in their modes of membrane association and cargo recognition. Here, we describe formation of hybrid coats between purified SNX-BARs, Snx3, and their cargos. Hybrid coats assemble at variable subunit ratios and diameters and show greater membrane-scaffolding activity than homogeneous coats. In vivo, Snx3 and

SUBMITTER: Navin Gopaldass 

PROVIDER: S-SCDT-10_1038-S44318-026-00716-0 | biostudies-other |

REPOSITORIES: biostudies-other