Deep single-cell decoding of human pancreatic islets reveals T2D β-cell gene expression defects

ABSTRACT: Pancreatic islets maintain glucose homeostasis through coordinated action of endocrine and affiliate cell types and are central to type 2 diabetes (T2D) genetics and pathophysiology. Our understanding of robust human islet cell type-specific alterations in T2D remains limited. Here, we report comprehensive single-cell transcriptome profiling of 245,878 human islet cells from 48 donors spanning non-diabetic, pre-diabetic, and T2D states, and we identify 14 distinct cell types detected in every donor. Cell-cluster analysis reveals ~25-30% -cell reductions consisting of -cell loss and proportional increases in a senescent -cell subpopulation in T2D donors, consistent with previous reports. Further, comparative data integration identifies 511 differentially expressed genes (DEGs) in T2D -c

SUBMITTER: Michael, L Stitzel 

PROVIDER: S-SCDT-10_1038-S44318-026-00744-W | biostudies-other |

REPOSITORIES: biostudies-other