Ki-67 shapes the nucleolus by anchoring chromatin via its amphiphilic properties

ABSTRACT: The nucleolus, a membrane-less organelle essential for ribosome biogenesis, adopts variable shapes across cell types and in response to environmental conditions, yet the mechanisms regulating its mor-phology and functional implications remain unclear. Using a high-throughput screen, we identify the proliferation marker Ki-67 as a central regulator of nucleolar shape. Ki-67 localises to the chromatin-nucleolus interface, where its depletion induces nucleolar rounding and reduces chromatin enrichment both at the nucleolar rim and within internal invaginations. This effect is driven by Ki-67's am-phiphilic properties conferred by two distinct affinity domains separated by a spacer. Given that chromatin loss is a common feature of rounded nucleoli in our screen, and acute chromatin digestion a

SUBMITTER: Daja Schichler 

PROVIDER: S-SCDT-10_1038-S44318-026-00747-7 | biostudies-other |

REPOSITORIES: biostudies-other