One-step generation of T-cell receptor knock-in mice in the TCRβ locus

ABSTRACT: Transgenic mouse models expressing predefined T-cell receptors (TCRs) have been instrumental in advancing our understanding of T-cell biology. However, these traditional models rely on random genomic insertion of large constructs, require labor-intensive embryo manipulation, and frequently result in aberrant TCR expression and phenotypes. These limitations render traditional models insufficient to meet the mounting demands for rapid and precise model systems to evaluate TCR specificities. To address these challenges, we developed in this study a streamlined method that uses adeno-associated virus (AAV) and CRISPR/Cas9-mediated genome editing to precisely integrate pre-rearranged TCR/ sequences into the mouse TCRβ (Trb) locus, enabling the rapid generation of TCR knock-in mice with physio

SUBMITTER: Jana Bilanovic 

PROVIDER: S-SCDT-10_1038-S44318-026-00779-Z | biostudies-other |

REPOSITORIES: biostudies-other