Atg18 interaction positions Atg2 for efficient lipid transfer into phagophore elongation

ABSTRACT: During macroautophagy, the de novo formation of the autophagosome at a membrane contact site (MCS) with the endoplasmic reticulum requires directional lipid flux for the growth of the initial phagophore before its sealing into an autophagosome and subse-quent fusion with the lysosome/vacuole. It remains unclear, however, how the formation of this specialized MCS and the directionality of the lipid flux are controlled. Here, we present the structure of the key lipid transfer protein Atg2 from yeast solved together with its Atg18 binding partner, a phosphatidylinositol-3-phosphate (PtdIns3P) effector, us-ing cryo-electron microscopy. We reveal a new interface in Atg2 that, together with PtdIns3P, is required for Atg18 recruitment and lipid transfer activity. Furthermore, we visualize lipid d

SUBMITTER: Sabrina Chumpen Ramirez 

PROVIDER: S-SCDT-10_1038-S44318-026-00802-3 | biostudies-other |

REPOSITORIES: biostudies-other