A coordinated transcriptional program controls de novo Golgi biogenesis

ABSTRACT: The Golgi apparatus expands during differentiation and increased secretory demand, yet the transcriptional mechanisms governing its biogenesis remain poorly defined. To investigate this process, we developed an enzymatic approach to ablate preexisting Golgi and induce large-scale de novo organelle formation. Transcriptional profiling of cells rebuilding the Golgi revealed a coordinated induction of a broad gene network, coinciding with structural and functional maturation of the organelle. This network spans all Golgi subcompartments and activities, supporting the existence of a unified "Golgi regulon" that synchronizes expression of components required for organelle integrity. Failure to activate this regulon impaired Golgi reassembly. Promoter analysis and RNAi screening identified CREB3

SUBMITTER: Francesca Forno 

PROVIDER: S-SCDT-10_1038-S44318-026-00828-7 | biostudies-other |

REPOSITORIES: biostudies-other