Mechanical forces regulate embryonic stem cell division in the developing brain

ABSTRACT: Research on mitosis has predominantly examined cell-autonomous mechanisms, yet its regulation and consequences within the broader physiological context remain largely unexplored. It remains therefore unknown whether and how spindle assembly and chromosome segregation are influenced by tissue properties. We have investigated this question in the mammalian embryonic brain, using high resolution microscopy combined with pharmalogical perturbations and minimal cell systems to break down the contribution of tissue environment to mitotic fidelity. We found that cell density imposes biomechanical constraints upon the apical radial glial (aRG) cell population during their highly proliferative period, which enhances spindle pole microtubule polymerization rates and potentiates chromosome mis-segreg

SUBMITTER: Veronique Marthiens 

PROVIDER: S-SCDT-10_1038-S44318-026-00848-3 | biostudies-other |

REPOSITORIES: biostudies-other