Nested TAD hierarchy defines cohesion zones on replicated chromosomes

ABSTRACT: During each cell cycle, cells must not only duplicate the DNA sequence but also preserve the three-dimensional genome architecture required for gene regulation and DNA repair. After replication, this requires coordination between cohesin-mediated loop extrusion and sister-chromatid cohesion for homology-directed DNA repair. Because extrusion promotes sister resolution whereas cohesion can impede extrusion, how both activities coexist on replicated chromosomes is unclear. Here we show that cohesin function is partitioned within the nested hierarchy of topologically associating domains (TADs): cohesive cohesin accumulates at high-level boundaries to form "cohesion zones," while loop-extruding cohesin occupies boundaries across all hierarchical levels. Our data support a model in which this s

SUBMITTER: Zsuzsanna Takacs 

PROVIDER: S-SCDT-10_1038-S44318-026-00902-0 | biostudies-other |

REPOSITORIES: biostudies-other