Switch from canonical to recombination-dependent DNA synthesis by M-CDK phosphorylation of DNA Pola

ABSTRACT: Cells preserve genome stability while occasionally generating genetic diversity through mutations and chromosomal rearrangements, but how these opposing outcomes are balanced remains unclear. Because late-replicating regions evolve more rapidly, we investigated the consequences of reduced origin licensing and lengthened S phase in yeast. Under these conditions, cells progress slowly through S phase without checkpoint activation, and enter mitosis with under-replicated DNA. We show that mitotic cyclin-dependent kinase (M-CDK) phosphorylates DNA polymerase α (Pol α), displacing it from replication forks and triggering fork collapse at mitotic entry. The resulting DNA damage response provides a window for mitotic DNA synthesis (MiDAS), enabling completion of replication through error-prone, r

SUBMITTER: Juan de Dios Barba Tena 

PROVIDER: S-SCDT-10_1038-S44318-026-00919-5 | biostudies-other |

REPOSITORIES: biostudies-other