Electrostatic control of chromatin compaction safeguards against apoptotic DNA release

ABSTRACT: Apoptosis involves extensive intracellular reorganisation to facilitate the clearance of dying cells. A key step in this process is the destruction of the genome through fragmentation by caspase-activated DNase (CAD). Rather than dispersing after CAD-mediated cleavage, DNA fragments remain compacted within a dense chromatin compartment, but the underlying mechanism and biological relevance of this compaction remain unknown. Here we show that global deacetylation of histone tails promotes chromatin compaction during apoptosis, preventing DNA release into apoptotic extracellular vesicles. Using synthetic effectors that modulate nucleosome electrostatics independently of histone modifications, we demonstrate that electrostatic attraction alone is sufficient to compact and sequester fragmented

SUBMITTER: Maximilian, F. D. Spicer 

PROVIDER: S-SCDT-10_1038-S44318-026-00932-8 | biostudies-other |

REPOSITORIES: biostudies-other