Dynamic engagement of dual-role regulators by the Sin3 HDAC complex

ABSTRACT: Gene expression is governed by dynamic transitions between repressive and activating transcriptional states. Chromatin readers and transcription factors play important roles in mediating these transitions, but how they assemble with regulatory machineries to enable crosstalk between repression and activation remains unclear. Here, we use an integrative structural approach, combining cryo-EM, crosslinking mass spectrometry, fragment-resolved protein interactome mapping and crystallography to determine how the dual-role chromatin reader Cti6 and the transcription factors Ash1 and Ume6 engage the Sin3 histone deacetylase complex, a major regulatory hub. We find that Cti6 and Ash1 engage a shared peripheral module, with Ash1 binding introducing a conformational rearrangement of Cti6, whereas U

SUBMITTER: Julien Olivet 

PROVIDER: S-SCDT-10_1038-S44318-026-00934-6 | biostudies-other |

REPOSITORIES: biostudies-other