1,10-phenanthroline inhibits sumoylation and reveals that yeast SUMO modifications are highly transient

ABSTRACT: The steady state levels of protein sumoylation depend on relative rates of conjugation and desumoylation. Whether SUMO modifications are generally long-lasting or short-lived is unknown. Here we show that treating budding yeast cultures with 1,10-phenanthroline abolishes most SUMO conjugations within one minute, without impacting ubiquitination, an analogous post-translational modification. 1,10-phenanthroline inhibits formation of the E1~SUMO thioester intermediate, demonstrating that it targets the first step in the sumoylation pathway. SUMO conjugations are retained after treatment with 1,10-phenanthroline in yeast that express a defective form of the desumoylase Ulp1, indicating that Ulp1 is responsible for eliminating existing SUMO modifications almost instantly, when de novo sumoylat

SUBMITTER: J., Bryan McNeil 

PROVIDER: S-SCDT-10_1038-S44319-023-00010-8 | biostudies-other |

REPOSITORIES: biostudies-other